Mélisande BLEIN-NICOLAS

Mélisande BLEIN-NICOLAS

 dernières mises à jour 04/01/2023 -   01 69 15 68 06
 melisande.blein-nicolas@inrae.fr
 Plateforme d'Analyse Protéomique de Paris Sud-Ouest
 Biologie de l'Adaptation et Systèmes en Évolution
 INRAE, Ingénieure de Recherche
 proteomics, mass spectrometry, integrative biology, systems genetics, quantitative genetics
 publications - orcid 

Position and education

  • 2022-present: Scientist-in-charge of the [PAPPSO proteomics facility] ( http://pappso.inrae.fr/en   )

  • 2015-present: INRAE research engineer, PAPPSO, UMR GQE-Le Moulon, Gif-sur-Yvette, France

  • 2010-2015: Post-doctoral fellow, INRAE, UMR GQE-Le Moulon, Gif-sur-Yvette, France

    • “Analysis of heterosis for protein abundance under normal and drought conditions in sunflower” (PIA Sunrise)
    • “Genome wide association study for protein expression under normal and water deficit conditions in maize leaves” (PIA Amaizing)
    • “Analysis of heterosis for protein abundance in inter- and intra-specific yeast hybrids grown under grape must fermentation” (ANR HeterosYeast)

  • 2008-2010: Postdoctoral fellow, CIRAD, UMR Biologie et Génétique des Interactions Plante-Parasite, Montpellier, France

    • “Functional validation of the role of CIGR1 in the resistance of rice to blast (Magnaporthe oryzae)

  • 2004-2007: PhD student (MENRT fellowship), IGEPP   (formely UMR Amélioration des Plantes et Biotechnologies Végétales), Rennes, France

    • Histological and transcriptomic analyses of the role of the Pch1 gene in the resistance of bread wheat to eyespot Oculimacula yallundae

  • 2004: Master of Science and Engineer degrees in Plants Genetics and Breeding, Agrocampus Ouest (formerly École Nationale Supérieure Agronomique de Rennes).

Activities of research and development

As plant geneticist and scientist-in-charge of the PAPPSO proteomics facility, I am developing high-throughput plant proteomics approaches to ease the use of proteomics in genetic studies that require the analysis of large cohort of samples.

Regarding my research activities, I am interested in understanding how living organisms respond and adapt to environmental changes. I focused on the identification of genes and molecular mechanisms responsible for the variation of adaptative traits, such as plant resistance to pathogenic fungi, yeasts ability to complete grape juice fermentation or drought tolerance in plants. During my PhD and my first post-doctoral fellowship, I addressed this issue by using functional approaches. Since I joined the UMR GQE-Le Moulon, I moved toward systems approaches based on the integration of multi-omics data to decipher the genotype/phenotype relationship. My current research activities are focused on the genetic control and plasticity of the molecular networks involved in the elaboration of ecophysiological traits related to drought tolerance in maize. This research takes place within the French investment for the future Amaizing program   .

Teaching and training

  • Introduction to mass-spectrometry based proteomics (Licence 1 and Master 2 at Université Paris Saclay)
  • Proteomics data analysis with the MCQ package (PAPPSO training sessions)

Publications

  • Tarkowski Łukasz P., Clochard T., Blein-Nicolas M. , Zivy M., Baillau T., Abadie C., Morère-Le Paven MC., Limami AM., Tcherkez G., Montrichard F.. (2024) The nitrate transporter-sensor MtNPF6.8 regulates the branched chain amino acid/pantothenate metabolic pathway in barrel medic (Medicago truncatula Gaertn.) root tip. Plant Physiology and Biochemistry, (206) 108213
  • Chasseriaud L., Albertin W., Blein-Nicolas M. , Balliau T., Zivy M., Coulon J., Bely M.. (2023) Physical Contact between Torulaspora delbrueckii and Saccharomyces cerevisiae Alters Cell Growth and Molecular Interactions in Grape Must. Beverages, 3 (9) 81
  • Duruflé H., Balliau T., Blanchet N., Chaubet A., Duhnen A., Pouilly N., Blein-Nicolas M. , Mangin B., Maury P., Langlade NB., Zivy M.. (2023) Sunflower Hybrids and Inbred Lines Adopt Different Physiological Strategies and Proteome Responses to Cope with Water Deficit. Biomolecules, 7 (13) 1110
  • Jamet E., Esquerré-Tugayé MT., Gallardo-Guerrero K., Rolland N., Zivy M., Blein-Nicolas M. , Vincent D., Gontero B., Rajjou L.. (2023) Obituary: Dominique Job (1947-2022). Front. Plant Sci., (14) 1188766
  • Prunier G., Cherkaoui M., Lysiak A., Langella O., Blein-Nicolas M. , Lollier V., Benoist E., Jean G., Fertin G., Rogniaux H., Tessier D.. (2023) Fast alignment of mass spectra in large proteomics datasets, capturing dissimilarities arising from multiple complex modifications of peptides. BMC Bioinformatics, 1 (24) 421
  • Baudouin E., Puyaubert J., Meimoun P., Blein-Nicolas M. , Davanture M., Zivy M., Bailly C.. (2022) Dynamics of Protein Phosphorylation during Arabidopsis Seed Germination. International Journal of Molecular Sciences, 13 (23) 7059
  • Millán-Oropeza A., Blein-Nicolas M. , Monnet V., Zivy M., Henry C.. (2022) Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance. Proteomes, 1 (10) 2
  • Plancade S., Berland M., Blein-Nicolas M. , Langella O., Bassignani A., Juste C.. (2022) A combined test for feature selection on sparse metaproteomics data—an alternative to missing value imputation. PeerJ, (10) e13525
  • Bassignani A., Plancade S., Berland M., Blein-Nicolas M. , Guillot A., Chevret D., Moritz C., Huet S., Rizkalla S., Clément K., Doré J., Langella O., Juste C.. (2021) Benefits of Iterative Searches of Large Databases to Interpret Large Human Gut Metaproteomic Data Sets. J. Proteome Res.,
  • Urrutia M., Blein-Nicolas M. , Prigent S., Bernillon S., Deborde C., Balliau T., Maucourt M., Jacob D., Ballias P., Bénard C., Sellier H., Gibon Y., Giauffret C., Zivy M., Moing A.. (2021) Maize metabolome and proteome responses to controlled cold stress partly mimic early-sowing effects in the field and differ from those of Arabidopsis. Plant Cell Environ,
  • Blein-Nicolas M. , Negro SS., Balliau T., Welcker C., Cabrera-Bosquet L., Nicolas SD., Charcosset A., Zivy M.. (2020) A systems genetics approach reveals environment-dependent associations between SNPs, protein coexpression, and drought-related traits in maize. Genome Res., 11 (30) 1593-1604
  • Belouah I., Nazaret C., Pétriacq P., Prigent S., Bénard C., Mengin V., Blein-Nicolas M. , Denton AK., Balliau T., Augé S., Bouchez O., Mazat JP., Stitt M., Usadel B., Zivy M., Beauvoit B., Gibon Y., Colombié S.. (2019) Modeling Protein Destiny in Developing Fruit. Plant Physiology, 3 (180) 1709-1724
  • Belouah I., Blein-Nicolas M. , Balliau T., Gibon Y., Zivy M., Colombié S.. (2019) Peptide filtering differently affects the performances of XIC-based quantification methods. J Proteomics, (193) 131-141
  • Henry C., Haller L., Blein-Nicolas M. , Zivy M., Canette A., Verbrugghe M., Mézange C., Boulay M., Gardan R., Samson S., Martin V., André-Leroux G., Monnet V.. (2019) Identification of Hanks-Type Kinase PknB-Specific Targets in the Streptococcus thermophilus Phosphoproteome. Front. Microbiol., (10) 1329
  • Langella O., Valot B., Balliau T., Blein-Nicolas M. , Bonhomme L., Zivy M.. (2017) X!TandemPipeline: A Tool to Manage Sequence Redundancy for Protein Inference and Phosphosite Identification. J. Proteome Res., 2 (16) 494-503
  • Millan-Oropeza A., Henry C., Blein-Nicolas M. , Aubert-Frambourg A., Moussa F., Bleton J., Virolle MJ.. (2017) Quantitative Proteomics Analysis Confirmed Oxidative Metabolism Predominates in Streptomyces coelicolor versus Glycolytic Metabolism in Streptomyces lividans. J. Proteome Res., 7 (16) 2597-2613
  • Blein-Nicolas M. , Zivy M.. (2016) Thousand and one ways to quantify and compare protein abundances in label-free bottom-up proteomics. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 8 (1864) 883-895